Ventura Institute of Bio Sciences
 
 
 
Biotechnology - Training - Ventura Institute of Bio Sciences
Ventura Institute of Bio Sciences
       
  Module Duration
» PG Diploma 1 year Click for more details
» Advance Diploma 180 days Click for more details
» Advanced Techniques 7 days Click for more details
» GEL Electrophoresis Techniques 7 days Click for more details
» Screening of Transgenic
Plants using PCR
7 days Click for more details
 
 
P.G DIPLOMA
Duration - 1 year

MODULE I

  1. INTRODUCTION TO GENERAL MICROBIOLOGY
  1. BASIC CONCEPTS   
    1. Sterilization techniques
    2. Microbiological culture media and its importance
    3. Isolation of microorganisms from soil air and water
    4. Pure culture preparation
    5. Staining techniques
  1. BIOCHEMICAL TESTS
  1. GROWTH CURVE
  1. INDUCTION OF MUTATIONS
    1. Physical mutagenesis
    2. Chemical mutagenesis
  1. MUTATIONAL STUDIES BY REPLICA PLATING
  1. BACTERIAL CONJUGATION
  1. SCREENING FOR DESIRED ORGANISMS
  1. ISOLATION AND IDENTIFICATION OF FUNGI
  1. PRODUCTION OF SECONDARY METABOLITES
  1. ANALYSIS OF METABOLITES
    1. Chromatography
    2. Solvent extraction
  1. ANTIBIOTIC ASSAY
  1. TITRIMETRIC ESTIMATION OF ORGANIC ACID
  1. SPECTROPHOTOMETRIC ESTIMATION OF ENZYME PROTEIN
  1. MICROBIAL ANALYSIS OF MILK
  1. WATER ANALYSIS

MODULE II:

  1. INTRODUCTION TO INDUSTRIAL MICROBIOLOGY
  1. BASIC CONCEPTS
    1. Sterilization of fermentation equipment
    2. Fermentation systems
    3. Steps in fermentation
    4. Designing of production media
    5. Development and transfer of seed
  1. SCREENING OF DESIRED ORGANISMS
  1. TYPES OF FERMENTATIONS
    1. Solid state fermentation
    2. Submerged fermentation
  1. DOWN STREAM PROCESSING
    1. CELL SEPERATION TECHNIQUES
    2. EXTRACTION OF DESIRED PRODUCTS
    3. PURIFICATION OF PRODUCTS
  1. ANALYSIS OF METABOLITES OR PRODUCTS
    1. Chromatography
    2. Spectrophotometric determination
    3. Titrimetric estimation
    4. Electrophoresis
  1. WATER ANALYSIS
  1. ENZYME PRODUCTION
    1. Solid sate fermentation
    2. Submerged fermentation
  1. ENZYME PURIFICATION TECHNIQUES
    1. Precipitation methods
  1. CHROMATOGRAPHY
  1. ENZYME ASSAY
  1. QUANTITATIVE ESTIMATION OF PROTEINS
  1. ENZYME KINETICS
    1. pH
    2. Temperature
    3. Substrate concentration
    4. Activators
    5. Inhibitors
  1. ELECTROPHORESIS TECHNIQUES
    1. SDS-PAGE
    2. Native PAGE
    3. Zymography
  1. IMMOBILIZATION ENZYMES

MODULE III:

RECOMBINANT DNA TECHNOLOGY

  1. FUNDAMENTALS
    1. Isolation of Genomic DNA
    2. Isolation of Total RNA
    3. Quantitative and Qualitative analysis of Nucleic acids
    4. Transformation
    5. Isolation of Plasmid DNA
    6. Agarose Gel Electrophoresis
    7. Restriction Digestion
    8. PCR Techniques

MODULE IV:

  1. INTRODUCTION TO IMMUNOLOGY
  1. AGGLUTINATION REACTIONS                        
    1. Blood grouping and Rh typing
    2. Rapid plasma reagin test (VDRL)
    3. Pregnancy test
  1. GEL DIFFUSION TECHNIQUES
    1. Ouchterlony Double Diffusion (ODD)
    2. Single Radial Immuno Diffusion (RID)
  1. IMMUNO ELECTROPHORESIS
    1. Counter Current Electrophoresis (CCE)
    2. Rocket Immuno Electrophoresis (RIE)
  1. ISOLATION OF IgG FROM BLOOD
  1. PURIFICATION OF IgG
    1. Salt precipitation
    2. Chromatography
    3. Quantitative estimation of proteins
    4. SDS PAGE
  1. SEPERATION OF LYMPHOCYTES
  1. ELISA (STRIP TEST)
  1. RAISING OF POLYCLONAL ANTIBODIES
  1. MONOCLONAL ANTIBODY PRODUCTION

MODULE V:

  1. INTRODUCTION TO MEDICAL BIOTECHNOLOGY
  1. BASIC CONCEPTS
  1. ISOLATION OF PATHOGENS
  1. BIOCHEMICAL TESTS (specialized test if any)
  1. ANTIBIOTIC SENSITIVITY
  1. DIAGNOSTIC TESTS
    1. C - reactive protein (CRP)
    2. Rapid Plasma Reagin (RPR)
    3. VDRL
    4. WIDAL
  1. REVIEW OF ANALYSIS
  1. REPORT PREPARATION
  1. BIOCHEMICAL TESTS
    1. Blood grouping and Rh typing
    2. Hemoglobin test
    3. Pregnancy test
  1. URINE ANALYSIS
    1. Detection of urine protein
    2. Detection of urine glucose
    3. Detection of ketone bodies in urine sample
  1. WBC Count
  1. MIC and MLC
  1. INTRODUCTION TO BIOINFORMATICS
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ADVANCE DIPLOMA
Duration - 180 Days (6 months)

MODULE I:

  1. INTRODUCTION TO GENERAL MICROBIOLOGY
  1. BASIC CONCEPTS
    1. Sterilization techniques
    2. Microbiological culture media and its importance
    3. Isolation of microorganisms from soil air and water
    4. Pure culture preparation
    5. Staining techniques
  1. BIOCHEMICAL TESTS
  1. GROWTH CURVE
  1. MUTATIONAL STUDIES BY REPLICA PLATING
  1. BACTERIAL CONJUGATION
  1. SCREENING FOR DESIRED ORGANISMS
  1. ISOLATION AND IDENTIFICATION OF FUNGI
  1. PRODUCTION OF SECONDARY METABOLITES
  1. ANTIBIOTIC ASSAY

MODULE II

  1. INTRODUCTION TO INDUSTRIAL MICROBIOLOGY
  1. BASIC CONCEPTS
    1. Sterilization of fermentation equipment.
    2. fermentation systems steps in fermentation
    3. designing of production media
  1. SCREENING OF DESIRED ORGANISMS.
  1. TYPES OF FERMENTATIONS
    1. solid state fermentation
    2. submerged fermentation
  1. DOWN STREAM PROCESSING
    1. Cell separation techniques
    2. Extraction of desired products
    3. Purification of products
  1. ANALYSIS OF METABOLITES OR PRODUCTS
    1. Chromatography
    2. Spectrophotometric determination

MODULE III

  1. INTRODUCTION TO ENZYMOLOGY
  1. ENZYME PRODUCTION
    1. solid state fermentation
    2. submerged fermentation
  1. ENZYME PURIFICATION
    1. salt precipitation
    2. Chromatography
  1. QUANTITATIVE ESTIMATION OF ENZYME PROTEINS
  1. ENZYME KINETICS
    1. pH
    2. Temperature
    3. Substrate concentration
  1. SDS-PAGE
  1. Zymography (theory)

MODULE IV

  1. INTRODUCTION TO IMMUNOLOGY
  1. GEL DIFFUSION TECHNIQUES
    1. Ouchterlony Double Diffusion (ODD)
    2. Single Radial Immuno Diffusion (RID)
  1. IMMUNO ELECTROPHORESIS
    1. Counter Current Electrophoresis (CCE)
    2. Rocket Immuno Electrophoresis (RIE)
  1. ISOLATION OF IgG FROM BLOOD
  1. PURIFICATION OF IgG
    1. Salt precipitation
    2. Chromatography
    3. Quantitative estimation of proteins
  1. SEPARATION OF LYMPHOCYTES
  1. AGGLUTINATION REACTIONS              
    1. Blood grouping and Rh typing
    2. Rapid plasma reagin test (VDRL)
    3. Pregnancy test
  1. ELISA (STRIP TEST)

MODULE V

INTRODUCTION TO r-DNA TECHNOLOGY

  1. ISOLATION OF GENOMIC DNA
    1. Plant
    2. animal
  1. ISOLATION OF TOTAL RNA
  1. QUALITATIVE AND QUANTITATIVE ANALYSIS OF NUCLEIC ACIDS
  1. PCR TECHNIQUES
  1. ISOLATION OF PLASMID DNA
  1. AGAROSE GEL ELECTROPHORESIS
  1. RESTRICTION DIGESTION
  1. TRANSFORMATION
  1. LIGATION
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ADVANCED TECHNIQUES
Duration - 7 Days

TECHNIQUES INVOLVED IN RAPD ANALYSIS

  1. PLANT DNA ISOLATION
  1. PURIFICATION OF ISOLATED DNA
  1. PCR AMPLIFICATION
  1. AGAROSE GEL ELECTROPHORESIS
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GEL ELECTROPHORESIS TECHNIQUES
Duration - 7 days
  1. AGAROSE GEL ELECTROPHORESIS
  1. SDS-POLY ACRALAMIDE GEL ELECTROPHORESIS
  1. NATIVE-POLY ACRALAMIDE GEL ELECTROPHORESIS
  1. IMMUNOELECTROPHORESIS
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SCREENING OF TRANSGENIC PLANTS USING PCR
Duration - 7 days
  1. DNA ISOLATION OF TRANSGENIC PLANT
  1. PURIFICATION OF DNA
  1. PCR AMPLIFICATION
  1. AGAROSE GEL ELECTROPHORESIS
 
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